Thymosin beta-4 is a very large molecule. In fact, it is so large that it cannot fit entirely into the receptor. Different sections of the molecule have different activities. TB-500 is the part of thymosin beta-4 hormone which promotes the most useful effects (overall healing, repair, new blood and muscle cells). For medical applications it is more practical to use the TB-500 instead of the entire Thymosin Beta-4 protein.
TB-500 MIXING, DOSING AND USE
Type of water to be used with TB-500:
Plain sterile water is the most suitable diluent for TB-500. Alternatively it can be reconstituted with sterile saline (0.9% NaCl) or sterile bacteriostatic water (0.9% sodium chloride). Plain sterile water should be readily available to buy without prescription in any local pharmacy. Alternatively it can also be purchased online. It is even available on ebay.
The insulin type syringe with 100 markings on the side should be used. They are readily available in any local pharmacy store.
How to mix (reconstitute) the TB-500:
Pull 1ml of water into the syringe and inject it into the vial with powder. You should never shake the vial when mixing. You should not inject the water directly into the powder with force, but rather let it gently slide down the inside of the vial. If it bubbles up, you should put the vial in the refrigerator and leave it there for about 15-30 minutes. The bubbles will be gone by then. You should then gently rotate the vial between your fingers until all of the powder has dissolved (it takes about 3-4 minutes).
The vials are under vacuum, so before you can take the tb-500 out, you need to release the vacuum. Take a fresh syringe, pull air into it and inject the air into the vial (not into liquid, but into air above the liquid). This will get rid of the vacuum. You can then pull out the reconstituted tb-500 as needed.
TB-500 Injection spot and procedure:
The tb-500 has a systematic effect regardless of where it is injected. Some believe that thymosin beta-4 should be injected as close to the injury as possible however there is no evidence to show this would be superior. It can be injected subcutaneously (stomach fat) or intramuscularly (shoulders, thighs, buttocks). Injections should be given in different sites (rotated) each time. Depending on the spot, you can either feel nothing or you can feel slight pain - you will learn your favorite spots in time.
Prior to injecting clean the injection spot as well as the vial rubberstopper with an alcohol swab. Make sure there are no air bubbles in the syringe. For subcutaneous injection fully insert the needle at an 45 degree angle, for intramuscular insert it at 90 degrees angle.
TB-500 Storage temperatures:
Before reconstitution (lyophilized / freeze dried powder):
refrigerated between 2 - 8 degrees celsius (36 - 46 Fahrenheit) it's good for 24 months
at room temperature (up to 37 degrees Celsius or 98.6 Fahrenheit) it's good for over 90 days
up to 45 degrees Celsius or 113 Fahrenheit it's good for about a week
After reconstitution (liquid):
liquid TB-500 should be refrigerated between 2 - 8 degrees celsius (36 - 46 Fahrenheit) at all times. Do not freeze it.
reconstituted TB-500 should be used within 8 days
TB-500 dosage and cycle duration:
Dosage depends on the purpose and severity of the injury / damage you are treating. People generally use between 4 to 8 mg of TB500 per week during the initial (loading) period of 4 to 6 weeks. Afterwards some opt to maintain the effects with a low 2 to 6 mg dose once every 2 weeks. The effects of TB-500 wear off within 2 - 3 weeks of injection.
1. TB-500 loading phase:
duration: between 4 - 6 weeks
dosage: between 4 - 8 mg of TB-500 per week
frequency of injection:2 mg per injection, between 2 - 4 times per week (depending on the total weekly dosage)
2. TB-500 maintainance phase:
duration: as long as needed
dosage: between 2 - 6 mg of TB-500 per 2 weeks
frequency of injection:2 mg per injection, between 2 - 3 times per 2 weeks (depending on the total bi-weekly dosage)